Coding
Part:BBa_K2271104:Design
Designed by: Jan Maika Group: iGEM17_Cologne-Duesseldorf (2017-10-27)
PEX13 membrane anchor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 32
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The membrane domain PEX13 (amino acids 200-310) was used as a peroxisomal membrane anchor for fluorescence proteins. We therefore added a triple GGGGS linker to allow an undisturbed c-terminal fusion.
Source
UniProtKB - P80667 (PEX13_YEAST)