Composite

Part:BBa_K2235011

Designed by: Shivashree Dhanaraj and Shanlin Tong   Group: iGEM17_Stockholm   (2017-10-11)
Revision as of 10:37, 28 October 2017 by Tongshl (Talk | contribs) (→‎Usage and Biology)


Sialidase enzyme coding composite N terminally attached to secretion system type 1

Usage and Biology

We used an already existing biobrick for HylA E.coli secretion system (BBa_K1166002) to secrete our sialidase from the iGEM 2017 distribution kit.

Characterization

Important Parameter

Purification and identification

Ligation of sialidase insert into secretion device

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3178
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3117
    Illegal XhoI site found at 127
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 649
    Illegal NgoMIV site found at 739
    Illegal AgeI site found at 2952
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1119
    Illegal SapI site found at 2934


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics/production
Parameters
None