Regulatory

Part:BBa_K2244006

Designed by: Chen Hong   Group: iGEM17_SSTi-SZGD   (2017-10-22)
Revision as of 08:17, 28 October 2017 by YuhangYang (Talk | contribs) (→‎Usage and Biology)

ColE promoter


Co1E promoter is derived from the promoter region of colicin E gene located in the ColE1 plasmid of E.coli. ColE promoter contains a ‘SOS’ operator region that allows the binding of LexA protein to repress transcription.


Usage and Biology

ColE promoter is 151bp in length. We used a light-repressed system containing a single light repressor LexA-VVD, and a ColE promoter that contains a SOS operator sequence for LexA binding to regulate the expression of target genes. The picture shows a colony PCR product of ColE promoter in pLEV1(408) vector.

Reference

Tomizawa, J. 1985. Control of ColE1 plasmid replication: initial interaction of RNA I and the primer transcript is reversible.Cell. 40(3):527-35.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None