Generator

Part:BBa_K2486004

Designed by: Cauă Westmann and Gabriel Lencioni Lovate   Group: iGEM17_USP-Brazil   (2017-10-22)
Revision as of 04:49, 28 October 2017 by Gabriellovate (Talk | contribs)


dtxR generator

The BBa_K486004 is a dtxR constitutive generator. This part is composed of:

  1. A promoter (BBa_J23108) from Anderson promoter collection.
  2. A ribosome binding site (BBa_B0034).
  3. A short spacer of 6bp (gtccta).
  4. dtxR gene (BBa K2486005).
  5. A short spacer of 16bp (ccagctcagtccgcat).
  6. A synthetic terminator (BBa_K2486006).

REFERENCES:

Boyd, J., Oza, M. N., & Murphy, J. R. (1990). Molecular cloning and DNA sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae. Proceedings of the National Academy of Sciences, 87(15), 5968-5972.

De Zoysa, A., Efstratiou, A., & Hawkey, P. M. (2005). Molecular characterization of diphtheria toxin repressor (dtxR) genes present in nontoxigenic Corynebacterium diphtheriae strains isolated in the United Kingdom. Journal of clinical microbiology, 43(1), 223-228.

https://www.wikigenes.org/e/gene/e/2649688.html

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None