Part:BBa_K2360009
rrnb' terminater+RcnR+pRcnR +SRRz+rrnb terminater
It is a high-efficiency nickel detecting device. This device consist two parts, the lysis module (BBa_K2360000) and nickel detecting module(BBa_K2360004).The lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of the engineering bacteria in different concentration of metal ions to prove its function. We can just transform the plasmid to E.coil BL21 to detect the nickel ion.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 296
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Figure 1. Bio-circuit of BBa_K2360009
Experiment
Lysis gene expression
we measured the growth curve of our engineering bacteria in different concentration of nickel ions.It was a pity that we found the OD600 of E. coli with did not decline after we added Ni2+, which showed this devices did not work as we expected.
Figure 2. the growth curve of nickel detecting device in different concentration of nickel ions.
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