Part:BBa_K2360011
rrnB' terminater+PbrR+pPbrR+SRRz+rrnB terminater
It is a high-efficiency lead detecting device. This device consist two parts, the lysis module (BBa_K2360000) and lead detecting module(BBa_K2360006).The lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of the engineering bacteria in different concentration of metal ions to prove its function. We can just transform the plasmid to E.coil BL21 to detect the lead ion.
Figure 1. Bio-circuit of BBa_K2360011
Our device has an obvious contact to lead ions’ concentration.we measured the growth curve of our engineering bacteria in different concentration of lead ions to prove its function.
Figure 2. the growth curve of lead detecting device in different concentration of mercury ions.
As shown in the growth curves of E. coli with lead detecting device, the growth rate of E. coli BL21 is decline obviously after adding the concentration of Pb2+ from 10-7M to 10-5M, indicating that this device can detect the lead ions.
Our device has an obvious contact to lead ions’ concentration. By detecting the shading of the product’s color, we can qualitatively detect the concentration of lead metals.
Figure 3. the comparison between ONPG and X-gal.c. lead detecting device’s chromogenic reaction at 20 mins.
We verified the detecting devices specificity of lead ions.The result indicated that the lead detecting device has good specificity for specific metal ions.
Figure 4. The growth of the E. coli with lead detecting device after adding different metal ions and ddH2O
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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