Composite

Part:BBa_K2302011

Designed by: Hongyang Wei   Group: iGEM17_NEFU_China   (2017-10-19)
Revision as of 01:22, 28 October 2017 by Zxjjdldb (Talk | contribs)


T7-His-Lpp-ompA-L-FABP-T7terminater

This plasmid cords for fatty acid binding protein and anchor it on the outer membrane, it consists four basic parts: promoter T7, RBS, Lpp-OmpA, and L-FABP. Lpp-OmpA is sequence cords for a functional peptide, which give efficient surface anchoring of FABP, and FABP is a kind of protein which can bind long chain fatty acid. By transforming this plasmid, a bacteria can catch long chain fatty acid in liquid and carry it on its membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 491
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716


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Categories
Parameters
None