Composite

Part:BBa_I751101

Designed by: Kenichiro Iwasaki   Group: iGEM07_Tokyo_Tech   (2007-10-23)
Revision as of 18:45, 24 October 2007 by Iwasaki (Talk | contribs)

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J540140dPr+hybrid promoter(plux-lac)

This part is made for B-side part in our project.

This part has a hybrid promoter, plux-lac. This promoter accepts two inputs. One is repression of lac repressor, and the other is activation of AHL.

GFP is assigned in the downstream of this hybrid promoter. We used this part for testing the hybrid promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
    Illegal BamHI site found at 1116
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1900


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