Translational_Unit

Part:BBa_K2212005

Designed by: Yuya Zhao   Group: iGEM17_UC_San_Diego   (2017-10-20)
Revision as of 23:38, 27 October 2017 by Yuz358 (Talk | contribs)


LAI w/ pconII+RiboswitchF+DYKDDDDK+rrnBT1

This part contains coding sequence for enzyme L-arabinose isomerase (EC 5.3.1.4), pconII promoter, riboswitch F, DYKDDDDK-tag, rrnB T1 terminator. The unlabeled sequences are random filler sequences. The protein sequence is originated from Arabidopsis thaliana. The protein coding sequence was codon optimized for Synechocystis sp. PCC 6803.

This part is intended to be used in Synechococcus elongatus PCC 7942 in the pathway for the production of tagatose from sucrose. Originally, this protein that mediates the bioconversion of galactose into tagatose as well as that of arabinose to ribulose.

Figure 1: Proposed tagatose synthesis pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1267
    Illegal NgoMIV site found at 1433
    Illegal AgeI site found at 41
    Illegal AgeI site found at 1366
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 374


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Categories
Parameters
None