Composite

Part:BBa_I751021

Designed by: Kenichiro Iwasaki   Group: iGEM07_Tokyo_Tech   (2007-10-23)
Revision as of 17:26, 24 October 2007 by Iwasaki (Talk | contribs)

plac-lux-cI-mCherry This is made for A-side plasmid in our project.

Regulated by lac repressor, this part makes LuxI, cI repressor, and mCherry (for detecting).

If needed, you can move this part into B-side plasmid (by DraIII or AatII), regulating the rate of cI/lac repressor.

This part is for our second plan, in case BBa_I751011 doesn't work.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2762
    Illegal suffix found in sequence at 5246
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2762
    Illegal SpeI site found at 5247
    Illegal PstI site found at 5261
    Illegal NotI site found at 2768
    Illegal NotI site found at 5254
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2762
    Illegal BglII site found at 3016
    Illegal BglII site found at 3698
    Illegal BamHI site found at 2798
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2762
    Illegal suffix found in sequence at 5247
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2762
    Illegal XbaI site found at 2777
    Illegal SpeI site found at 5247
    Illegal PstI site found at 5261
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2003
    Illegal SapI site found at 920


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