Part:BBa_K2305000
strong promoter, cycle amplification system and GFP/RFP dual fluorescent system
Strong constitutive promoter pcat and promoter plux stars the circuit, Luxl catalyzes the production of AHL, which will combine with LuxR. Their compound will promote the expression of plux. The Lacl will inhibit the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, with the amplified expression of GFP on the contrary.
Agarose gel electrophoresis
We calculated the new part’s base number to be 4739bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
DNA sequencing
The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1591
Illegal BglII site found at 2770 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4451
Illegal AgeI site found at 4563 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 64
Illegal BsaI.rc site found at 3449
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