Part:BBa_J61002

EX-Ptet-S-rbsRFP-P "RFP reporter" This part has been improved by iGEM 2017 K2205026

Plasmid for construction of promoter basic parts and their derivatives. This plasmid can be used two ways:

1. Insertion of a promoter element between XbaI and SpeI sites results in a RFP reporter while retaining the ability to do biobrick assembly. Part J61002 is the tet promoter variant of the plasmid.
2. Insertion of a protein generating device or RNA gene (cutting the part with XbaI/PstI, inserting into SpeI/PstI of J61002) results in a standard pSB1A2 plasmid containing an r0040.yourpart composite.

See similar part J61003 for a similar method of construction for constructing open reading frame parts, or r0040.b0034.your ORF composite parts in pSB1A2. The same procedure can be used with part J23019 for the same effect in a pSB3C6 plasmid backbone.

• RS 19:58, 24 November 2008 (UTC): The original entry of this part included both a plasmid and plasmid backbone. It has been split into a plasmid backbone and part. The physical Registry distribution has been updated accordingly.

The original part is now BBa_J61002-R0040.

ApE file:{pBca1020-R0040}

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2927
  Illegal XbaI site found at 2942
  Illegal SpeI site found at 2
  Illegal PstI site found at 885
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2927
  Illegal SpeI site found at 2
  Illegal PstI site found at 885
  Illegal NotI site found at 878
  Illegal NotI site found at 2933
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2927
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2927
  Illegal XbaI site found at 2942
  Illegal SpeI site found at 2
  Illegal PstI site found at 885
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2927
  Illegal XbaI site found at 2942
  Illegal SpeI site found at 2
  Illegal PstI site found at 885
  Illegal AgeI site found at 581
  Illegal AgeI site found at 693
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1966