Reporter

Part:BBa_K2305011

Designed by: PENG ZHAO   Group: iGEM17_BIT   (2017-10-19)
Revision as of 19:05, 27 October 2017 by Buqi (Talk | contribs)


GFP/RFP dual fluorescent system with pbad

GFP/RFP dual fluorescent system with pbad

Agarose gel electrophoresis

We calculated the new part’s base number to be 4352bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

K230500

DNA sequencing

The figure 2 is a comparison with the results of the sequencing. It verified the accuracy of the sequence further

K230500
K230500
K230500
K230500


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2383
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 4064
    Illegal AgeI site found at 4176
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3062
    Illegal SapI site found at 961


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Categories
Parameters
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