Composite

Part:BBa_K2360000:Design

Designed by: LIAO PEILIN   Group: iGEM17_SCUT-China_A   (2017-10-27)
Revision as of 18:47, 27 October 2017 by NANZHUCHEN (Talk | contribs) (→‎Design Notes)


SRRz+rrnb terminater


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The SRRz gene codes maybe three protein S,R,Rz.The product of S gene would cause lesions on the cytoplasmic membrane through which the product coded by the R gene escapes to the periplasm and causes murein-degrading, while the Rz gene’s product may be an endopeptidase that can cleave the oligopeptide crosslinks in the peptidoglycan and/or between peptidoglycan and the outer membrane. The expression of this gene finally cause the lysis of E.coil. We use it as a lysis module to make a high-efficiency biosensor.

Source

phage

References