Device

Part:BBa_K2205005

Designed by: Bradley Brown, Sophie Badger, Marcia Pryce   Group: iGEM17_Newcastle   (2017-09-21)
Revision as of 17:45, 27 October 2017 by M00 (Talk | contribs)


Fim Standby Switch

The Fim switch is a processing module for the Sensynova framework, it is designed to continuously express the red chromoprotein eforRed (K592012) to indicate that the part is functional. The FimE protein (K137007) flips a 300bp promoter region which switches expression from eforRed to rhlI (J64718). This produces the quorum sensing molecule C4-AHL which can be used to activate downstream reporter cells.

--T--Newcastle--MP--Expression_of_GFP_Fim.png

Expression of sfGFP over a standard growth curve. The Fim switch strain was co-cultured with the reporter strain (K2205015) which expresses sfGFP in response to C4-AHL. FimW indicates a white Fim switch reporter in the [ON] state, where the promoter region produces C4-AHL. FimR indicates a red Fim switch reporter in the native [OFF] state which does not produce C4-AHL. Each data point it the mean of 3 biological replicates.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None