Part:BBa_K2203000
gamS protein
GamS is a protein that protects linear DNA templates from degradation in a cell- free environment. It is used to enhance cell-free protein synthesis by inhibiting exonuclease activity.
This part has a demonstrated positive effect on protein synthesis in a cell-free chassis. GamS can be added to a cell-free reaction system in several ways: By adding it in its synthesized protein form, or by adding simply its coding sequence to the cell-free reaction, or (in the case of a self-made cell lysate) by making the lysate from cells which were transformed with the GamS-plasmid. For the first and last example, see experimental results below.
Characterization of lysate expressing the gamS protein
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<figcaption>Figure 1: Protein expression in M15-T7 lysate.</figcaption>
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Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 298
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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