Composite

Part:BBa_K2253002

Designed by: Surta Dave   Group: iGEM17_Austin_UTexas   (2017-10-27)
Revision as of 17:03, 27 October 2017 by Registry (Talk | contribs)

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Optimized Blue Chromoprotein

As previously stated, this is the original blue chromoprotein sequence (BBa_K592009) that has been codon optimized. The part has been made a composite part with the promotor and RBS sequence, BBa_K608002 in the pSB1C3 biobrick backbone. Codon optimization allows it to function more efficiently in E.coli, therefore creating a lower metabolic burden on the cells that it is encoded in. When these sequence is put into a vector and transformed into a plasmid it shows a blue phenotype. Codon optimization also makes this part more stable than the original, making It a good candidate to use as a biosensor for things like toxins such as lead.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 694
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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