Regulatory

Part:BBa_K2275005

Designed by: Wan-Wen Ting   Group: iGEM17_NCKU_Tainan   (2017-10-09)
Revision as of 16:59, 27 October 2017 by TedShihncku (Talk | contribs)

PyeaR(BBa_K216005) with additional NsrR binding site in front of B0030 RBS

We add an extra NsrR binding sequence before RBS which is expected to decrease the basal level of expression on the original part of K381001 in order to improve function of BBa_K381001.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 107
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None