Coding
Part:BBa_K2205004
Designed by: Jessica Birt, Sophie Badger Group: iGEM17_Newcastle (2017-09-21)
T7-Sarcosine Oxidase
Sarcosine Oxidase under the control of an IPTG-inducible T7 promoter. Catalyses sarcosine to yield glycine, formaldehyde and hydrogen peroxide.
Formaldehyde Production
Sarcosine Oxidase Degradation of Sarcosine to Formaldehyde: Lysed cell cultures induced with 40 µl of a 100 mM stock of IPTG tested with formaldehyde strips. From left to right: SOX+0 µl sarcosine, SOX+IPTG+0 µl sarcosine, WT+0 µl sarcosine, WT+IPTG+0 µl sarcosine, SOX+50 µl sarcosine, SOX+IPTG+50 µl sarcosine, WT+50 µl sarcosine, WT+IPTG+50 µl sarcosine, SOX+200 µl sarcosine, SOX+IPTG+200 µl, WT+200 µl sarcosine, WT+IPTG+200 µl.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
| None |