Coding

Part:BBa_K2271067:Design

Designed by: Jason Marcel Müller, Manuel Lentzen   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 16:27, 27 October 2017 by Registry (Talk | contribs)

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PEX5 variant R19


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1341
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1128
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1633
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutagenesis


Source

ATGGACGTAGGCTCTTGCAGCGTTGGGAACAACCCGCTGGCTCAGTTGCACAAGCACACTCAACAGAATAAGTCTTTGCAGTTCAATCAAAAAAACAACGGCCGTCTAAATGAATCCCCGCTACAGGGGACGAATAAGCCCGGCATCTCAGAGGCGTTTATCTCCAACGTAAATGCCATCTCACAAGAGAACATGGCTAATATGCAAAGGTTCATAAATGGGGAACCCTTGATTGATGATAAGCGTCGTATGGAGATAGGACCGTCTTCTGGGAGACTTCCTCCTTTTAGTAATGTACATTCCTTACAAACCTCCGCTAACCCTACTCAGATAAAGGGCGTCAATGATATAAGCCACTGGAGTCAGGAATTTCAGGGAAGCAACTCAATTCAGAACAGGAATGCCGATACGGGTAATTCCGAGAAAGCCTGGCAGAGAGGCTCAACGACTGCGAGTTCACGTTTTCAGTATCCGAATACTATGATGAACAATTACGCCTATGCAAGTATGAACTCACTTTCCGGTTCCCGTTTGCAGTCACCGGCCTTCATGAATCAACAGCAGAGCGGGAGAAGTAAAGAAGGTGTGAATGAGCAAGAACAGCAACCTTGGACCGACCAATTCGAGAAGTTGGAAAAAGAGGTCAGTGAAAACCTGGACATCAACGACGAAATCGAGAAAGAAGAAAATGTCTCTGAAGTAGAACAGAATAAGCCCGAGACTGTTGAAAAGGAGGAGGGAGTCTACGGCGACCAATACCAGTCTGATTTCCAAGAGGTATGGGACTCAATTCACAAGGACGCGGAAGAGGTCTTACCATCAGAACTGGTAAACGATGACCTAAACCTAGGCGAGGACTATCTAAAATATTTAGGCGGGCGTGTGAATGGCAACATTGAGTATGCCTTTCAATCTAATAATGAGTATTTTAACAACCCAAATGCATATAAAATAGGCTGCCTATTAATGGAAAATGGCGCAAAACTAAGCGAAGCCGCACTTGCGTTCGAAGCGGCCGTCAAGGAGAAGCCAGATCATGTAGACGCCTGGTTAAGATTAGGATTGGTGCAAACGCAAAATCACAAAACTCTAAATGGTATATCCGCACTAGAGGAATGTTTAAAACTGGATCCTAAAAACTTGGAGGCCATGAAGACTTTAGCTATATCTTACATCAACGAAGGATATGACATGAGCGCATTTACAATGTTAGATAAGTGGGCTGAAACAAAATACCCGGAAATATGGTCCAGGATCAAACAGCAAGACGATAAGTTTCAAAAGGAGAAGGGTTTCACACACATCGACATGAATGCCCACATCACAAAGCAATTCTTGCAGCTAGCCAACAACTTATCCACGATAGACCCTGAAATACAACTATGTTTAGGATTGCTATTTGCTACCAAGGATGATTTCGATAAAACAATAGACTGCTTCGAAAGCGCTCTGCGTGTGAATCCCAATGACGAACTTATGTGGAATCGTCTGGGCGCTTCACTTGCGAACTCTAACCGTTCAGAAGAGGCTATACAGGCCTACCACAGGGCTCTACAGTTAAAGCCCAGCTTCGTCCGTGCAAGATACAATCTAGCGGTATCATCTATGAACATTGGATGCTTCAAAGAGGCGGCCGGCTACTTGCTTTCCGTACTAAGCATGCATGAAGTTAATACCAACAACAAAAAAGGTGACGTAGGTTCACTTCTTAACACGTATAACGACACGGTGATCGAAACCCTGAAAAGGGTCTTCATCGCGATGAATCGTGATGACCTACTACAAGAAGTAAAACCTGGGATGGACTTAAAGCGTTTCAAAGGAGAATTTTCATTTTAA

References