Device

Part:BBa_K2325101

Designed by: Wenyong Lou   Group: iGEM17_SCUT-FSE-CHINA   (2017-10-16)
Revision as of 15:42, 27 October 2017 by Tamce (Talk | contribs)


Formamidase expression device

The BBa_K2325101 contains formamidase gene with T7 promoter and terminator.

Usage and Biology

BBa K2325101--1.png
Figure 1. Growth curves of E.coli containing formamidase gene in a basal MOPS medium in the presence of formamide as the nitrogen source comparing with negative controls

E. coli containing formamidase gene, reached maximum concentration after 48 hours in the basal MOPS medium in the presence of 200 mM formamide. While E.coli without formamidase gene basically did not grow.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 189
    Illegal NgoMIV site found at 676
    Illegal NgoMIV site found at 871
    Illegal NgoMIV site found at 1018
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None