Reporter

Part:BBa_K2492000

Designed by: ZHOU Xinyu   Group: iGEM17_SUSTech_Shenzhen   (2017-09-21)
Revision as of 15:21, 27 October 2017 by X Maze (Talk | contribs)


GFP (optimised, for use in the C. elegans chassis)

Modified GFP with three introns which can help well expression of GFP in C.elegans. In our group, we use it to indicate the location of neuron AWB in C.elegans. We integrated str1::CoCHR::GEM-GECO::GFP transgene in C.elegans by microinjection. Modified GFP will be highly expressed in AWB driven by promoter str1(no biobrick part).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 264
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 797


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