Composite

Part:BBa_K2407307

Designed by: Xinyu Chen   Group: iGEM17_Tianjin   (2017-10-12)
Revision as of 15:12, 27 October 2017 by 01is01 (Talk | contribs) (→‎Introduction)

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TDH3 Promoter + vox + Ura + Ura3 terminator + vox + β-carotene

Introduction

It is regulated by TDH3 promoter. This composite workes for inserting the vox sites and Ura3 gene between them can be replaced by any structural gene. Initially, Before the vika-vox system works, the β-carotene does not express. After mating, the component between two vox sites will be deleted by vika enzyme and the β-carotene gene will synthesize β-carotene. Yeasts turns to orange.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2410
    Illegal BglII site found at 4097
    Illegal XhoI site found at 2109
    Illegal XhoI site found at 2241
    Illegal XhoI site found at 3060
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2277
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1390
    Illegal BsaI site found at 5001
    Illegal BsaI site found at 7356
    Illegal BsaI.rc site found at 1837
    Illegal BsaI.rc site found at 7687
    Illegal SapI site found at 4401
    Illegal SapI.rc site found at 1237


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