Part:BBa_K2462007

Sensor of halide+dehalogenase

RdhANP is a kind of dehalogenase first discovered in Nitratireductor.pacificus and it has been considered as one of the most promising enzymes to deal with the organic halide in wastewater due to its unparalleled specialty working in oxygenic conditions. This year we plan to use RdhANP as the cleaner of the wastewater and try our best to make the management more suitable in reality. We get an inspiration from Peking-China’s work in 2013. In part BBa_J61051, they designed a circuit which can sense salicylate and induce the following reactions for its degradation. Through their results, we get the information that this sensor can also function when other substances like CL-PHL, which can serve as a substrate for our enzyme RdhANP. Thus, we decide to make our brick which combines both RdhANP and BBa_J61051 and only when the substrates are detected, enzyme will be produced. It’s necessary to improve the accuracy of the project and release the burden of the bacteria. However, problems still remain. That is, this device has little capacity to resist the disturbance of other substances which can give the sensor a stronger message to produce enzymes. Thus, only when the substrates of RdhANP occupy large proportion can this device exploit its advantage. And we plan to solve this problem by eliminating this interference before the management of organic halide using the device described by Peking-China to turn substance like salicylate into intermediates of TCA cycle.

Related part: Part:BBa K2462000 - parts.igem.org https://parts.igem.org/Part:BBa_K2462000

Original Part:BBa J61051 - parts.igem.org https://parts.igem.org/Part:BBa_J61051

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 786
  Illegal BglII site found at 1952
  Illegal BglII site found at 3176
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 77
  Illegal NgoMIV site found at 618
  Illegal NgoMIV site found at 1364
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1507