Translational_Unit

Part:BBa_K2387078

Designed by: Bart Scholten   Group: iGEM17_Wageningen_UR   (2017-10-27)
Revision as of 14:45, 27 October 2017 by Registry (Talk | contribs)

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TEV cleavable CpxA-eYFP[1-154]-nZIP and CpxA-eYFP[155-238]-cZIP+ araC/pBAD promoter

In order to visualize Cpx pathway activation, transmembrane signal transducing protein CpxA is fused to respectively eYFP(1-154) and N-Leucine Zipper [1] ,as well as eYFP(155-238) and C-Leucine Zipper [2]. CpxA and eYFP[1-154] /eYFP[155-238] are fused with a TEV cleavable peptide. When cotransformed with CpxR-TEV protease fusion activation of the Cpx pathway is visualized in combination with CpxA-eYFP[1-154]-N-Leucine Zipper fusion due to eYFP reassembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
None