Reporter

Part:BBa_K2268003

Designed by: Devin Camenares   Group: iGEM17_Kingsborough_NY   (2017-10-27)
Revision as of 13:18, 27 October 2017 by Registry (Talk | contribs)

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p(cI)-LacZ alpha

This is the LacZ alpha fragment (BBa_I732006) under the control of a cI responsive promoter (BBa_R0051). To be used in conjunction with a part that expressed the cI repressor (or a chassis that contains the repressor, such as a strain with the pDawn plasmid).

LacZ alpha fragment can associate with the omega fragment, if present, to restore beta-galactosidase activity to a cell. This can be visualized as a blue pigment color when cells are grown in the presence of X-gal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None