Coding

Part:BBa_K2268002:Design

Designed by: Devin Camenares   Group: iGEM17_Kingsborough_NY   (2017-10-27)
Revision as of 13:13, 27 October 2017 by Djcamenares (Talk | contribs)

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MazF-ssrA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was codon optimized, then further modified by removal of any ACA triplets so that the MazF mRNA is not a target of MazF activity


Source

Based upon genomic sequence from E. coli for MazF protein and ssrA tag. Similar to part BBa_K302033

References