Part:BBa_K116404:Experience

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By replacing the weaker RBS BBa_B0032 of the original part with a stronger RBS BBa_B0034, we have successfully constructed an improved phosphate sensor-GFP reporter BBa_K2447000 (https://parts.igem.org/Part:BBa_K2447000). Our part shows, on average, 40 fold increase in GFP expression when compared to the previous version of the construct. The original phosphate construct is also insensitive to high phosphate concentrations above 50 µM where similar levels of GFP expression are observed (Figure 4). Unlike the previous construct, our improved phosphate construct is much more sensitive to various phosphate concentrations from 0 to 1000 µM, particularly at phosphate concentrations above 50 µM.

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