Coding

Part:BBa_K2407301

Designed by: Xinyu Chen   Group: iGEM17_Tianjin   (2017-10-12)
Revision as of 10:28, 27 October 2017 by Linton (Talk | contribs)


Ura3 gene

This is the part regulatory region from the URA3 gene coding for OMP decarboxylase, an essential protein in the uracil synthesis pathway in S. cerevisiae budding yeast. It is widely used as a nutrition tag in Saccharomyces cerevisiae.

Description

URA3, a gene on chromosome V in Saccharomvces cerevisiae, is widely used in researches concerning yeasts as a “marker gene” (systematic name YEL021W. URA3) and used as a label for chromosomes or plasmids. URA3 encodes Orotidine 5'-phosphate decarboxylase—an enzyme that catalyzes one reaction in the synthesis of pyrimidine ribonucleotides. We obtained the URA3 gene from the PRS416 plasmid，which worked as a vector for our functional genes.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 669
    Illegal SapI.rc site found at 516


[edit]
Categories
//cds
Parameters
chassisSaccharomyces cerevisiae