Regulatory

Part:BBa_K2361010:Design

Designed by: Thijs Ettema   Group: iGEM17_Groningen   (2017-10-13)
Revision as of 10:13, 27 October 2017 by Matthijs Groningen (Talk | contribs) (→‎References)

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pUSP45 lactococcal promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This strong promoter can potentially be used to express the different CRISPR parts of the detection system.



Source

The promoter sequence was amplified from a gblock designed by the team.

References

Martien van Asseldonk, Ger Rutten, Marco Oteman, Roland J. Siezen, Willem M. de Vos and Guus Simons. 95 (1990) 155-160 Elsevier

Overkamp W, Beilharz K, Detert Oude Weme R, Solopova A, Karsens H, Kovács Á, Kok J, Kuipers OP, Veening JW. Appl Environ Microbiol. 2013 Oct;79(20):6481-90