Regulatory

Part:BBa_K2361008:Design

Designed by: Thijs Ettema   Group: iGEM17_Groningen   (2017-10-13)
Revision as of 10:09, 27 October 2017 by Matthijs Groningen (Talk | contribs) (→‎References)


p32 lactococcal promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This strong promoter can potentially be used to express the different CRISPR parts of the detection system.


Source

The promoter sequence was amplified from a MG36C plasmid obtained from the Department of Molecular Genetics, Nijenborgh 7, 9747 AG, the Netherlands.

References

Jos M. B. M. van der Vossen, Daniel van der Lelie, and Gerard Venema. Appl. Environ. Microbiol. Oct. 1987, p. 2452-2457 van de Guchte, M., van der Vossen, J.M.B.M., Kok, J. and Venema, G. (1989) Appl. Environ. Microbiol. 55, 224-228