Coding

Part:BBa_K2381002:Design

Designed by: Zhujun Xia   Group: iGEM17_HZAU-China   (2017-10-20)
Revision as of 08:21, 27 October 2017 by ShaneX995 (Talk | contribs) (→‎Design Notes)


Rad1+ pMagFast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1371
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2214
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to prevent the fusion protein has steric hindrance, We use a 12aa GS linker to link the light-induced dimerization protein with a part of dCas9, which split by a rational strategy of Cas9. According to the different function and spatial position between α-helix lobe and nuclease, we split dCas9 into two parts. Rad1 includes a-helix lobe, Rad2 includes nuclease lobe.

Source

no

References