T7

Part:BBa_K2382003

Designed by: TING-YU LIN   Group: iGEM17_CSMU_NCHU_Taiwan   (2017-10-26)
Revision as of 07:45, 27 October 2017 by Herlohuang (Talk | contribs)


T7 promoter & Lac operator and RBS from PET-29a

This part originated from pET-29 a (+) Vectors, and it is composed of T7 promoter, Lac operator, and RBS.because the original part has the restriction site”Xba1”in the sequence,and the sequence of this restriction site is” TCTAGA. “ We do the single mutation to convert TC”T”AGA to TC”C”AGA . Thus, it would not effect role to make the composition of our part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has lac operator . When we want to use it to produce protein, it could be induced by lac operator.

[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
Parameters
None