Coding

Part:BBa_K2381005:Design

Designed by: Zhujun Xia   Group: iGEM17_HZAU-China   (2017-10-20)
Revision as of 07:41, 27 October 2017 by ShaneX995 (Talk | contribs) (→‎Source)


cod + nMag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1740
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 403


Design Notes

In order to prevent the fusion protein has steric hindrance, We use a 12aa GS linker to link the light-induced dimerization protein with a part of dCas9, which split by a conventional strategy. According to the paper, this conventional strategy is effective by rapamycin induced.

Source

The light-induced protein nMagHigh is made from the VVD protein family derived from the fungus Neurospora crassa. The cod2 is made from the dCas9 Protein derived from the Streptococcus pyogenes.

References