Composite

Part:BBa_K2378004

Designed by: Muhammad Farhan Maulana, Marvin Nathanael Iman, Zulhaerana Bahar   Group: iGEM17_ITB_Indonesia   (2017-10-27)
Revision as of 07:16, 27 October 2017 by Registry (Talk | contribs)

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pSal + PETase Coding Device

ITB_Indonesia iGEM team 2017 designed a PETase enzyme coding device consisting of NahR-pSal regulatory part (BBa_J61051, https://parts.igem.org/Part:BBa_J61051) which is activated by salicylate inducer. In effect, PETase is only expressed when this device detect the presence of salicylate, which binds to NahR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1303
    Illegal NotI site found at 2212
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 77
    Illegal NgoMIV site found at 618
    Illegal AgeI site found at 1957
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None