Composite

Part:BBa_K2260001:Design

Designed by: Rachelle Varga   Group: iGEM17_Calgary   (2017-10-25)
Revision as of 07:05, 27 October 2017 by Atika ibrahim (Talk | contribs) (→‎Design Notes)


phaC1J4 w/ Histidine tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The phaCBA operon was codon optimized for use in E. coli using the codon optimization table. Furthermore, four restrictions sites present in the original operon obtained from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">BBa_K1149051</a> were removed, including any new restriction sites introduced from codon optimization. Lastly, his tags were added to the operon.

Source

Enter source part here.

References