Composite

Part:BBa_K2260000:Design

Designed by: Rachelle Varga   Group: iGEM17_Calgary   (2017-10-25)
Revision as of 06:59, 27 October 2017 by Atika ibrahim (Talk | contribs) (→‎Source)


PhaCBA operon w/ Histidine tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The phaCBA operon was codon optimized for use in E. coli using the codon optimization table. Furthermore, four restrictions sites present in the original operon obtained from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">BBa_K1149051</a> were removed, including any new restriction sites introduced from codon optimization. Lastly, his tags were added to the operon.

Source

The naturally existing phaCAB operon is present in R. eutropha H16. The operon sequence was taken from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">BBa_K1149051</a> and rearranged.

References