Coding
Part:BBa_K2325000
Designed by: Wenyong Lou Group: iGEM17_SCUT-FSE-CHINA (2017-10-14)
Formamidase (FOR)
The BBa_K2325000 contains a coding sequence of formamidase which catalyzes the conversion of formamide to ammonia.
Usage and Biology
Formamidase is an enzyme involved in the production of ammonia for providing nitrogen source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of nitrogen in E.coli.
formamidase gene
HCO - NH2 + H2O ⇒ HCOOH + NH3
Gel analysis
Enzymatic properties
Figure 1.Enzymatic activity of formamidase under different temperatures and pH
Figure 3.Influence of metal ion on the enzymatic activity of formamidase
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 69
Illegal NgoMIV site found at 556
Illegal NgoMIV site found at 751
Illegal NgoMIV site found at 898 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |