Coding

Part:BBa_K2381013

Designed by: YJ GENG   Group: iGEM17_HZAU-China   (2017-10-20)
Revision as of 04:00, 27 October 2017 by Johnson Huang (Talk | contribs)


nMag-LucC

this part can response to blue light (470nm), rapidly bind to the corresponding parts, BBa_K2381014, due to eletrostatic interaction and dimerize. Its cofactor for light sensing is flavin adenine dinucleotide (FAD). After dimerization the split luciferase will complement to recover its abillity to generate fluorescenece.

Fig.1 Prove the function of pMag & nMag. n=1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 762
    Illegal AgeI site found at 901
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 403


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Parameters
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