Coding

Part:BBa_K2309022:Design

Designed by: Yijing Gong   Group: iGEM17_XJTLU-CHINA   (2017-09-26)
Revision as of 03:04, 27 October 2017 by Zixin.Rong (Talk | contribs) (→‎Design Notes)


LL-37 with A His-Tag for Lactococcus lactis NZ9000 (codon optimized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The purpose of the LL-37 with 6x His-tag is used for purifying the anti-microbial peptide production. 6xHis tag is six histidine amino acids tandem together which has strong affinity with Ni-NTA purification system. After purification, using dot blot method to confirm the LL-37 were produced.

Source

Homo sapiens

References