DNA

Part:BBa_K2440016

Designed by: Qijie Xu   Group: iGEM17_NUDT_CHINA   (2017-10-22)
Revision as of 03:04, 27 October 2017 by Jhyzxqj (Talk | contribs)


miR-511 target sequence

It is the target sequence of miR-511, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-511 in an Ago2 dependent manner, that is, knockdown of miR-511 was achieved by transfecting cells with miRNA locker.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 4
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 4
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.

[edit]
Categories
Parameters
None