Composite

Part:BBa_K2328059

Designed by: Yiyuan Gao   Group: iGEM17_TJU_China   (2017-10-20)
Revision as of 01:29, 27 October 2017 by LLiu (Talk | contribs) (→‎Usage and Biology)

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nonsense sequence + Linker II + Linker.a

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 165
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 50


Usage and Biology

We use Linker II and Linker.a to separate smURFP from Acma. The meaningless sequence is 150 bp and has the restriction enzyme cutting sites of XhoI、BglII、NcoI、NheI.

Reference

[1]Yamamoto S, Wada J, Katayama T, Jikimoto T, Nakamura M, Kinoshita S, et al. (2010) Genetically modified Bifidobacterium displaying Salmonella-antigen protects mice from lethal challenge of Salmonella Typhimurium in a murine typhoid fever model. Vaccine 28: 6684–6691.

[2]Rodriguez EA,Tran GN , Gross LA, et al. A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein .[J].NATURE METHODS,2016:763-769

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