Part:BBa_K2419003

TagCFP for analysis of elastomeric tissue

This part can be used to analyze the quality i.e. of an elastomeric tissue. While such a tissue is formed, TagCFP as a fluorescent protein can be added. As a result, the maxima of emission and absorption of the fluorescent protein shift to a higher level. The emission maximum shift can only be ascribed to the assembly of CFP into the tissue. The shift can therefore be used as a qualitively screen of the tissue around the fluorescent protein. The tissue network can be characterized regarding its structure and density. These parameters will give information about the quality of the polymeric network.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization by the Franconia 2017 iGEM Team

TagCFP is a monomeric protein emitting bright cyan light. It is based on the natural fluorescence of the jellyfish Aequorea macrodactyla, which expresses a GFP-like fluorescent protein.

Figure 1: Fluorescence spectrum of TagCFP showing absorption and emission at variant wavelengths.

The results show a difference in the wavelength of the absorption and the emission. The absorption of EGFP has its maximum at a wavelength of 457.4 nm and the emission maximum lays at a wavelength of 478.1 nm. The overlap of absorption and emission can be seen in the area around 470 nm.

We further plotted the measured values of the excitation wavelength and the emission wavelength of EGFP to obtain a height profile given in the diagram below (Figure 2).

Figure 2: Excitation wavelength and emission wavelength of TagCFP.

The diagram displays the distribution of the different wavelengths. The red colored area reveals the overlap of excitation and emission at its uppermost distinctness.