Part:BBa_K2516003

Membrane-bound SuperNova (for C. reinhardtii)

This is a membrane-bound version of SuperNova protein, originally introduced by iGEM 2014 Carnegie Mellon team.

SuperNova is a photosensitizing protein, which produces Reaction Oxygen Species (superoxide and singlet oxygen) under irradiation of 500-600nm wavelength light. It is a monomeric version of another photosensitizing protein called KillerRed. There are several advantages over KillerRed, SuperNova does not oligomeraize inside the cell and does not interfere with mitotic cell division. This advantage allows performing targeted protein inactivation inside the cell by fusion to the target protein without loss of target protein function.

The second application that seems very simple and powerful is light controlled safety system. SuperNova can be utilized as a killer switch for genetically modified organisms. Under irradiation of 500-600nm wavelength light, SuperNova will generate ROS and kill the cell. Its phototoxicity was proven in bacterial and mammalian cells.

In case of KillerRed, it was

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 606
  Illegal BsaI.rc site found at 691