Device

Part:BBa_K2520020

Designed by: Noa Eden, Dana Kadosh   Group: iGEM17_TECHNION-ISRAEL   (2017-10-25)
Revision as of 18:59, 26 October 2017 by Danakd (Talk | contribs)


CMV-Mono Display:peanut 3-hGH

This device is a complete system for displaying proteins on cells' membrane. The Igk leader (BBa_K2520029) is a short signal peptide that prompts the translocation of a protein to the cellular membrane. PDGFR (BBa_K2520037) is a trans-membrane domain that anchors all the components located between the igk leader and the PDGFR itself to the membrane. His (BBa_K2520032) is a tag that binds to specific antibodies and provide an indirect method for verifying the display of proteins on the membrane. The protein that we chose to express on the membrane is an epitope that is known target of peanut allergy (BBa_K2520042, Arah2/6 protein) and is component related to our specific project. The CMV promoter (BBa_K1119006) is a constitutive expression promoter in mammalian cells and hGH (BBa_K404108) serves as a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
    Illegal BamHI site found at 730
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 679
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1372


[edit]
Categories
Parameters
None