Part:BBa_I742106

Sam8 gene with ribosome binding site

This part includes the sam8 gene of the bacterium Saccharothrix espanaensis DSM 4429 along with its native ribosome binding site. The coding sequence alone, with no ribosome binding site, as also been submitted as BBa_I742141. Sam8 encodes a tyrosine ammonia lyase which converts tyrosine to p-coumaric acid. This gene is naturally involved in caffeic acid biosynthesis (Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006)) and is part of the artifical vanillin biosynthesis pathway devised by the Edinburgh iGEM2007 team.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 808
  Illegal BamHI site found at 1210
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 551
  Illegal AgeI site found at 498
  Illegal AgeI site found at 667
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 102
  Illegal BsaI site found at 368