![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2275007
Designed by: Cheng-Yen Lin Group: iGEM17_NCKU_Tainan (2017-10-14)
Revision as of 17:19, 26 October 2017 by TedShihncku (Talk | contribs)
PlacI-B0034-NiR cluster
This composite part encodes nitrite reductase cluster (nirB and nirD) which derived from E. coli K-12 MG1655 strain. In order to achieve our goal of regulation, we choose lacI regulated promoter R0010 and B0034 RBS for high expression level. Then we used Griess reagent assay to test the activity of NiR
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 286
Illegal BglII site found at 1287
Illegal BglII site found at 2070
Illegal BamHI site found at 567 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2093
Illegal AgeI site found at 551
Illegal AgeI site found at 947
Illegal AgeI site found at 2105 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 401
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Categories
Parameters
None |