Composite

Part:BBa_K2407303

Designed by: Xinyu Chen   Group: iGEM17_Tianjin   (2017-10-22)
Revision as of 16:53, 26 October 2017 by Cccxyyyyyyyy (Talk | contribs) (→‎Introduction)


β-carotene gene

A composite part of three key genes to produce β-carotene which can be expressed in Saccharomyces Cerevisiae.

Introduction

After reviewing the literature, we understand that carotene can be overexpressed in Saccharomyces cerevisiae. Furthermore, carotenoid production levels were higher in strains containing integrated carotenogenic genes. Overexpression of crtYB and crtI from X. dendrorhous was sufficient to enable carotenoid production. crtYB, crtI, and crtE can complete the expression of β-carotene, and the cell is orange.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 749
    Illegal BglII site found at 2436
    Illegal XhoI site found at 448
    Illegal XhoI site found at 580
    Illegal XhoI site found at 1399
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 616
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3340
    Illegal BsaI site found at 5695
    Illegal BsaI.rc site found at 176
    Illegal BsaI.rc site found at 6026
    Illegal SapI site found at 2740


[edit]
Categories
//cds
Parameters
None