![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2275007
Designed by: Cheng-Yen Lin Group: iGEM17_NCKU_Tainan (2017-10-14)
Revision as of 16:50, 26 October 2017 by TedShihncku (Talk | contribs)
PlacI-B0034-NiR cluster
This composite part encodes nitrite reductase cluster (nirB and nirD) which derived from E. coli K-12 MG1655 strain. To achieve our goal of regulation, we choose lacI regulated promoter R0010 and B0034 RBS for high expression level .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2093
Illegal AgeI site found at 551
Illegal AgeI site found at 947
Illegal AgeI site found at 2105 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 401
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Categories
Parameters
None |