Coding

Part:BBa_K2368007:Design

Designed by: BIT-China 2017   Group: iGEM17_BIT-China   (2017-10-01)
Revision as of 16:16, 26 October 2017 by Hrlpl (Talk | contribs)

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PGal 1/10-T1R2-CYC1t


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 563
    Illegal BglII site found at 2061
    Illegal BglII site found at 2481
    Illegal BglII site found at 2643
    Illegal BamHI site found at 674
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 291
    Illegal AgeI site found at 1572
    Illegal AgeI site found at 2790
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We construct the part by Gibson assembly.And the Gal1/Gal10 promoter and the CYC1 terminator stem from the PESC-Ura plasmid. Please see more at the main page.

Source

References